試薬および製造原料の輸入販売 シングルセル解析


バイオマーカー

バックグランド

*

バイオマーカーはNIHにより「正常時あるいは病態時の生物学的変化、治療に対する薬理的応答に関して、客観的な測定と評価ができる指標」と定義されています。アクロバイオシステムズが提供するリコンビナントタンパク質にはEGFR, HER2, PCSK9, SOST, PDCD1, CD3, CD20, CD19といった、診断や治療のバイオマーカーとして有用なものがあります。これらの製品は疾病のメカニズムを解明する研究に広く使われています。

HER2

ヒト上皮成長因子受容体2(HER2)は受容体型チロシンキナーゼで、よく研究が進んでおりNeuまたはERBB2とも呼ばれます。 HER2の増幅や過剰発現による異常な活性化が乳がん患者の30%で認められており、再発や予後の不良に関係すると考えられています。

HER2はPI3K経路やRas/ERK経路といった下流のシグナルカスケードを調節することにより細胞の増殖および分化を制御します。

リコンビナントヒトHER2 タンパク質
[Thr 23- Thr 652 (Accession # AAA75493), Cat # HE2-H5225 ] はヒト293細胞(HEK293)から発現

純度:>95%
DTT還元SDS-PAGEによる

エンドトキシン: 1.0 EU/μg 未満
LAL法による

*

*

HER2, VEGF, IGF はがんの増殖および進行に関与します。

バイオマーカーを標的とするタンパク質製剤が、大腸がんの第Ⅱ相、第Ⅲ相臨床試験、および市販後臨床試験で評価されています。

*

rhHER2の生物活性 ELISAによる結合測定

PD-1 / PDCD1

PD-1(Programmed cell death protein 1)は、主にT細胞とプロB細胞の表面に発現します。PD-1は負の免疫応答を示し、炎症性サイトカインの産生とT細胞の増殖を抑制します。PD-1が機能するためには、リガンドであるPD-L1と結合することが必須であると考えられています。PD-1とPD-L1の相互作用が阻害されるとPD-1による免疫抑制が働きません。

近年、PD-1は複数のがんにおいて薬物ターゲットとして注目されています。PD-1のモノクローナル抗体(Nivolumab)が非小細胞肺ガン、悪性黒色腫、腎細胞ガンに有効であることが臨床試験で示されました。Nivolumabを投与された多くの患者で部分奏功から完全奏功と判定されました。

PD1とPD-L1の相互作用

*

ヒト
PD-1完全長リコンビナントタンパク質

[Pro 21 - Leu 288] (Accession # NP_005009.2) HEK293細胞を使って発現

DTTで還元したタンパク質はSDS-PAGEで45-60 kDaに検出される

*

完全長PD1の還元SDS-PAGE

製品名 製品コード EC50 (ug/ml)
rh Full length PD1 Protein PD1-HC214 0.13
rh PD1-ECD Protein PD1-H5221 1.93

完全長PD-1(rh full length PD1)と細胞外ドメインPD-1(rh PD1-ECD)のヒトPD-L1 Fc キメラに対するEC50の比較

*

完全長PD-1(rh full length PD1)と細胞外ドメインPD-1(rh PD1-ECD)のELISAでの結合活性の比較
完全長PD-1タンパク質(カタログ番号 PD1-HC214)で作製したELISA(2μg/ml、100μl/ウェル)により、ヒトPD-L1 Fc キメラ(カタログ番号 PD1-H5258)を20-200ng/ml の範囲で定量できた。

バイオマーカー

製品名 製品コード タグ ホスト 容量
Human ACE2 /ACEH AC2-H5251 C-Fc HEK293 20ug, 1mg
Human ACVR1b ACB-H5251 C-Fc HEK293 200ug, 1mg
Human ADAM12 AD2-H5228 C-His HEK293 50ug, 1mg
Human ADIPOQ /ACRP30 ADQ-H5229 C-His HEK293 100ug, 1mg
Human ADIPOQ /ACRP30 ADQ-H5250 C-Fc HEK293 100ug, 1mg
Human AGR2 /AG2 AG2-H5221 C-His HEK293 50ug, 1mg
Human ALK-1 /ACVRL1 AL1-H5227 C-His HEK293 100ug, 1mg
Human ALK-3 /BMPR1A AL3-H5228 C-His HEK293 200ug, 1mg
Human ALK-3 /BMPR1A AL3-H5259 C-Fc HEK293 200ug, 1mg
Human ALK-6 /BMPR1B AL6-H5226 C-His HEK293 50ug, 1mg
Human ANGPTL3 AN3-H5226 C-His HEK293 100ug, 1mg
Human ANGPTL4 AN4-H5243 N-His HEK293 100ug, 1mg
Human Apo-A1 AP1-H5225 C-His HEK293 50ug, 1mg
Human AZU1 / HBP AZ1-H5225 C-His HEK293 100ug, 1mg
Cynomolgus B2M B2M-C5226 C-His HEK293 100ug, 1mg
Human B2M B2M-H5225 C-His HEK293 100ug, 1mg
Rat B2M B2M-R5229 C-His HEK293 50ug, 1mg
Human B7H4 B74-H5222 C-His HEK293 100ug, 1mg
Human BACE1 BA1-H5213 Native HEK293 100ug, 1mg
Human BACE1 BA1-H5220 C-His HEK293 100ug, 1mg
Human BACE1 BA1-H5261 N-Fc HEK293 200ug, 1mg
Human BCL2L1 BC1-H513a N-GST E. Coli 100ug, 1mg
Human BCL2L2 BC2-H5122 C-His E. Coli 100ug, 1mg
Human CA10 / CARPX CAX-H5223 C-His HEK293 50ug, 1mg
Human CA3 CA3-H5120 C-His E. Coli 100ug, 1mg
Human CA4 CA4-H5223 C-His HEK293 50ug, 1mg
Human CADM1 CA1-H5225 C-His HEK293 100ug, 1mg
Human CAII /CA2 CA2-H5228 C-His HEK293 100ug, 1mg
Human CAIX /CA9 CA9-H5226 C-His HEK293 50ug, 1mg
Human Carboxypeptidase E CAE-H5225 C-His HEK293 100ug, 1mg
Mouse Carboxypeptidase E CAE-M5222 C-His HEK293 100ug, 1mg
Human CD300LG CDG-H5223 C-His HEK293 100ug, 1mg
Cynomolgus CD3E CDE-C5226 C-His HEK293 100ug, 1mg
Human CD3E CDE-H5286 C-Twin-Strep HEK293 25ug, 250ug
Human CDH6 /Cadherin-6 CA6-H5229 C-His HEK293 100ug, 1mg
Human CEACAM6 /CD66c CE6-H5223 C-His HEK293 100ug, 1mg
Human CEACAM8 /CD66b CE8-H5224 C-His HEK293 100ug, 1mg
Human CHI3L1 CH1-H5228 C-His HEK293 100ug, 1mg
Human CLEC3B CLB-H5226 C-His HEK293 100ug, 1mg
Human CLUS /Clusterin CLU-H5227 C-His HEK293 100ug, 1mg
Human CNDP1 CN1-H5224 C-His HEK293 50ug, 1mg
Human CNTFR CNR-H5225 C-His HEK293 100ug, 1mg
Human COMP / TSP5 COP-H5220 C-His HEK293 100ug, 1mg
Human CPA2 CP2-H5221 C-His HEK293 50ug, 1mg
Human CPA4 CP4-H5222 C-His HEK293 50ug, 1mg
Human CPM CPM-H5222 C-His HEK293 50ug, 1mg
Human CRP CRP-H5226 C-His HEK293 500ug, 5mg
Human CST1 CS1-H5225 C-His HEK293 20ug, 1mg
Human CST2 CS2-H5224 C-His HEK293 50ug, 1mg
Human CST4 CS4-H5223 C-His HEK293 100ug, 1mg
Human CST5 CS5-H5220 C-His HEK293 50ug, 1mg
Human CST7 CS7-H5222 C-His HEK293 50ug, 1mg
Human CTLA4 /CD152 CT4-H5229 C-His HEK293 200ug, 1mg
Human CTLA4 /CD152 CT4-H5284 C-Twin-Strep HEK293 25ug, 250ug
Human CTSD CTD-H5226 C-His HEK293 50ug, 1mg
Human CTSL1 CT1-H5222 C-His HEK293 50ug, 1mg
Human CTSS CTS-H5224 C-His HEK293 50ug, 1mg
Human DKK1 DK1-H5286 C-Twin-Strep HEK293 25ug, 250ug
Human DKK1 /DKK-1 DK1-H5221 C-His HEK293 100ug, 1mg
Human DKK3 /DKK-3 DK3-H5220 C-His HEK293 200ug, 1mg
Human DLL4 DL4-H5227 C-His HEK293 100ug, 1mg
Human DLL4 DL4-H5259 C-Fc HEK293 100ug, 1mg
Human DPP7 DP7-H5222 C-His HEK293 50ug, 1mg
Human ECAD /Cadherin-1 ECD-H5222 C-His HEK293 100ug, 1mg
Human EFNA1 EF1-H5251 C-Fc HEK293 100ug, 1mg
Human EFNB1 EF1-H5223 C-His HEK293 200ug, 1mg
Human EGFR /ErbB1 EGR-H5222 C-His HEK293 50ug, 1mg
Human EGFR /ErbB1 EGR-H5252 C-His HEK293 100ug, 1mg
Human EGFR /ErbB1 EGR-H5285 C-Twin-Strep HEK293 25ug
Mouse EGFR /ErbB1 EGR-M5224 C-His HEK293 50ug, 1mg
Human EPHA7 /EHK3 EP7-H5221 C-His HEK293 100ug, 1mg
Human EPHB4 EP4-H5229 C-His HEK293 200ug, 1mg
Human Ephrin A3 EA3-H5227 C-His HEK293 200ug, 1mg
Human Ephrin A3 EA3-H5258 C-Fc HEK293 200ug, 1mg
Human Ephrin A4 EA4-H5250 C-Fc HEK293 250ug, 1mg
Human EPOR EPR-H5253 C-Fc HEK293 100ug, 1mg
Mouse ErbB2 ER2-M5220 C-His HEK293 100ug, 1mg
Human ErbB-3 /HER3 ER3-H5259 C-Fc HEK293 200ug, 1mg
Human ErbB-3 /HER3 ER3-H5223 C-His HEK293 100ug, 1mg
Human ErbB-3 /HER3 ER3-H5288 C-Twin-Strep HEK293 25ug, 250ug
Human ErbB4 /HER4 ER4-H5221 C-His HEK293 100ug, 1mg
Human ErbB4 /HER4 ER4-H5251 C-Fc HEK293 100ug, 1mg
Human FABP1 FA1-H5148 N-His E. Coli 200ug, 1mg
Human FABP2 FA2-H5149 N-His E. Coli 200ug, 1mg
Human FABP3 /H-FABP FA3-H5128 C-His E. Coli 100ug, 1mg
Human FABP5 FA5-H5140 N-His E. Coli 100ug, 1mg
Human FABP6 FA6-H5141 N-His E. Coli 100ug, 1mg
Human FABP7 FA7-H5142 N-His E. Coli 200ug, 1mg
Human Fas Ligand FAL-H5241 N-His HEK293 50ug, 1mg
Human FCER1A FCA-H5228 C-His HEK293 100ug, 1mg
Human FGFBP1 FG1-H5225 C-His HEK293 50ug, 1mg
Human FGFRL1 FG1-H5250 C-Fc HEK293 50ug, 1mg
Human FOLR1 FO1-H5229 C-His HEK293 100ug, 1mg
Human FOLR1 FO1-H528b C-Twin-Strep HEK293 25ug, 250ug
Mouse FOLR1 FO1-M5223 C-His HEK293 100ug, 1mg
Human FOLR2 FO2-H5223 C-His HEK293 100ug, 1mg
Human FZD2 FZ2-H5251 C-Fc HEK293 100ug, 1mg
Human FZD5 FZ5-H5259 C-Fc HEK293 100ug, 1mg
Human FZD7 FZ7-H5258 C-Fc HEK293 100ug, 1mg
Human Galectin3 GA3-H5129 C-His E. Coli 200ug, 1mg
Human GFRA1 GF1-H5224 C-His HEK293 100ug, 1mg
Human GFRA1 GF1-H5252 C-Fc HEK293 100ug, 1mg
Human GFRA2 GF2-H5225 C-His HEK293 200ug, 1mg
Human GFRA2 GF2-H5253 C-Fc HEK293 100ug, 1mg
Human GHR GHR-H5257 C-Fc HEK293 50ug, 1mg
Human GHR /GHBR GHR-H5222 C-His HEK293 50ug, 1mg
Human GP73 /GOLM1 GO1-H5224 C-His HEK293 50ug, 1mg
Human GPA33 GP3-H5224 C-His HEK293 50ug, 1mg
Mouse GPA33 GP3-M5225 C-His HEK293 50ug, 1mg
Human GPC3 GP3-H5223 C-His HEK293 100ug, 1mg
Human GPNMB GPB-H5229 C-His HEK293 100ug, 1mg
Human Granzyme B GZB-H5225 C-His HEK293 20ug, 500ug
Human GRN GRN-H5229 C-His HEK293 100ug, 1mg
Human HE4 HE4-H5223 C-His HEK293 100ug, 1mg
Human HER2 HE2-H5287 C-Twin-Strep HEK293 25ug, 250ug
Human HER2 /ErbB2 HE2-H5225 C-His HEK293 100ug, 1mg
Human HER2 /ErbB2 HE2-H5253 C-Fc HEK293 100ug, 1mg
Human c-MET /HGFR MET-H5227 C-His HEK293 200ug, 1mg
Human c-MET /HGFR MET-H5256 C-Fc HEK293 200ug, 1mg
Human c-MET /HGFR MET-H5287 C-Twin-Strep HEK293 25ug, 250ug
Human HMGB1 HM1-H5220 C-His HEK293 100ug, 1mg
Human HSP90AA1 HS1-H511y Native E. Coli 100ug, 1mg
Human HSP90AA1 HS1-H513x N-GST E. Coli 100ug, 1mg
Human HVEM HVM-H5283 C-Twin-Strep HEK293 25ug, 250ug
Human HVEM /TNFRSF14 HVM-H5258 C-Fc HEK293 200ug, 1mg
Human IFNAR1 IF1-H5253 C-Fc HEK293 100ug, 1mg
Human IFNAR2 IF2-H5224 C-His HEK293 100ug, 1mg
Human IFNAR2 IF2-H5255 C-Fc HEK293 100ug, 1mg
Human IGF2BP2 IG2-H5228 C-His HEK293 50ug, 1mg
Human IGFBP3 IG3-H5229 C-His HEK293 100ug, 1mg
Human IGSF4B /SynCAM3 CA3-H5221 C-His HEK293 100ug, 1mg
Human IL13RA1 IL1-H5224 C-His HEK293 100ug, 1mg
Human IL18BP ILP-H5222 C-His HEK293 200ug, 1mg
Human IL18BP ILP-H5253 C-Fc HEK293 200ug, 1mg
Human IL1R2 /CD121b IL2-H4256 C-Fc HEK293 100ug, 1mg
Human IL1RAP ILP-H5225 C-His HEK293 200ug, 1mg
Human IL1RAP ILP-H5256 C-Fc HEK293 200ug, 1mg
Human IL1RL1 IL1-H5229 C-His HEK293 200ug, 1mg
Human IL1RL1 IL1-H5250 C-Fc HEK293 200ug, 1mg
Human IL27RA ILA-H5254 C-Fc HEK293 100ug, 1mg
Human IL6R /CD126 ILR-H4223 C-His HEK293 50ug, 1mg
Human INSR INR-H5220 C-His HEK293 100ug, 1mg
Human KIM1 /TIM1 KI1-H5227 C-His HEK293 100ug, 1mg
Human KIM3 /HAVCR2 KI3-H5252 C-Fc HEK293 100ug, 1mg
Human KIRREL1 KI1-H5225 C-His HEK293 100ug, 1mg
Human KLK1 KL1-H5223 C-His HEK293 50ug, 1mg
Human KLK13 KL3-H5225 C-His HEK293 50ug, 1mg
Human KLK3 KL3-H5226 C-His HEK293 50ug, 1mg
Human KLK6 KL6-H5222 C-His HEK293 100ug, 1mg
Human KLK7 KL7-H5228 C-His HEK293 50ug, 1mg
Human KLK8 KL8-H5229 C-His HEK293 50ug, 1mg
Human LDLR LDR-H5224 C-His HEK293 100ug, 1mg
Human LDLR LDR-H5281 C-Twin-Strep HEK293 25ug, 250ug
Human LRRC4 LR4-H5221 C-His HEK293 100ug, 1mg
Human LTA4H LTH-H5220 C-His HEK293 20ug, 500ug
Human MBL MBL-H5220 C-His HEK293 100ug, 1mg
Mouse mKlk1b22 KL2-M5224 C-His HEK293 50ug, 1mg
Human MSLN MSN-H5222 C-His HEK293 100ug, 1mg
Human MSLN MSN-H5223 C-His HEK293 100ug, 1mg
Human MSLN MSN-H526x N-Fc HEK293 100ug, 1mg
Human Mucin-1 /MUC1 MU1-H5252 C-Fc HEK293 100ug, 1mg
Human NLGN4X NLX-H5225 C-His HEK293 100ug, 1mg
Human Noggin /NOG NON-H5257 C-Fc HEK293 100ug, 1mg
Human NTRK2 / TRKB NT2-H5254 C-Fc HEK293 100ug, 1mg
Human NTRK2 /TRKB NT2-H5228 C-His HEK293 100ug, 1mg
Human NTRK3 /TrkC TRC-H5226 C-His HEK293 100ug, 1mg
Human NTRK3 /TrkC TRC-H5254 C-Fc HEK293 100ug, 1mg
Human PCSK9 PC9-H5213 なし (ネイティブ) HEK293 20ug, 500ug
Human PCSK9 PC9-H5223 C-His HEK293 50ug, 1mg
Human PCSK9 PC9-H5226 C-His HEK293 20ug, 500ug
Human PCSK9 PC9-H528c C-Twin-Strep HEK293 25ug, 250ug
Mouse PCSK9 PC9-M5228 C-His HEK293 100ug, 1mg
Cynomolgus PD1/PDCD1 PD1-C5254 C-Fc HEK293 100ug, 1mg
Human PD1 PD1-H5221 C-His HEK293 100ug, 1mg
Human PD1 PD1-H5257 C-Fc HEK293 100ug, 1mg
Human PD1 PD1-HC214 なし (ネイティブ) HEK293 20ug, 200ug
Human PD1/PDCD1 PD1-H5284 C-Twin-Strep HEK293 25ug, 250ug
Mouse PD1 /PDCD1 PD1-M5228 C-His HEK293 100ug, 1mg
Mouse PD1 /PDCD1 PD1-M5259 C-Fc HEK293 100ug, 1mg
Human PGF PGF-H5229 C-His HEK293 20ug, 500ug
Human PTH1R PTR-H5222 C-His HEK293 100ug, 1mg
Human REG4 RE4-H5221 C-His HEK293 100ug, 1mg
Human RENIN REN-H5221 C-His HEK293 100ug, 1mg
Mouse RENIN REN-M5222 C-His HEK293 100ug, 1mg
Human ROR1 RO1-H522y C-His HEK293 100ug, 1mg
Human ROR1 RO1-H5250 C-His HEK293 200ug, 1mg
Mouse ROR1 RO1-M5221 C-Fc HEK293 100ug, 1mg
Mouse ROR1 RO1-M5250 C-His HEK293 100ug, 1mg
Human ROR2 RO2-H5251 C-Fc HEK293 50ug, 1mg
Human R-Spondin-1 RS1-H4221 C-Fc HEK293 50ug, 1mg
Human R-Spondin-1 RS6-H4220 C-His HEK293 50ug, 1mg
Human R-Spondin 3 RS6-H5222 C-His HEK293 50ug, 1mg
Human RTN4R RTR-H5222 C-His HEK293 100ug, 1mg
Human S100A6 S16-H5111 C-His HEK293 100ug, 1mg
Human S100A8 S18-H5112 なし (ネイティブ) E. Coli 100ug, 1mg
Human SECTM1 SE1-H5227 なし (ネイティブ) E. Coli 100ug, 1mg
Human SERPINA1 SE1-H5226 C-His HEK293 50ug, 1mg
Human SERPINA3 SE3-H5221 C-His HEK293 50ug, 1mg
Human SERPINA8 SE8-H5227 C-His HEK293 50ug, 1mg
Human SERPIND1 / HCF2 HC2-H5224 C-His HEK293 50ug, 1mg
Human SERPINE2 SE2-H5225 C-His HEK293 25ug, 500ug
Human SERPINF1 SE1-H5221 C-His HEK293 100ug, 1mg
Human SERPINH1 SE1-H5224 C-His HEK293 50ug, 1mg
Human SFRP1 /SARP2 FR1-H5228 C-His HEK293 50ug, 1mg
Human SFRP4 SF4-H5224 C-His HEK293 50ug, 1mg
Human SIRPD SID-H5258 C-Fc HEK293 50ug, 1mg
Human SOST HST-H5245 N-His HEK293 100ug, 1mg
Human TCN2 TC2-H5221 C-His HEK293 100ug, 1mg
Human TGFBR2 TG2-H5252 C-Fc HEK293 100ug, 1mg
Human TIM3 /HAVCR2 TM3-H5229 C-His HEK293 100ug, 1mg
Human TIM3 /HAVCR2 TM3-H5258 C-Fc HEK293 100ug, 1mg
Human TMED4 TM4-H5228 C-His HEK293 50ug, 1mg
Human TMED4 TM4-H5259 C-Fc HEK293 100ug, 1mg
Human TNFRII TN2-H5227 C-His HEK293 50ug, 1mg
Human VEGFR1 /Flt-1 VE1-H5220 C-His HEK293 100ug, 1mg
Human VEGFR2 /KDR KDR-H5227 C-His HEK293 200ug, 1mg
Human VEGFR2 /KDR KDR-H5280 C-Twin-Strep HEK293 25ug, 250ug
Human VEGFR2 /KDR VLR-H5227 C-His HEK293 100ug, 1mg
このページのトップへ
お気軽にお問い合わせ・ご相談ください 受託開発サービスはこちら TEL:03-6277-3233 FAX:03-6277-3265 メールでのお問い合わせ:customer@bizcomjapan.co.jp

バイオ関連の受託開発サービス・試薬の輸入販売ならビジコムジャパンにお問い合わせください。お客様の用途やご要望に沿ったサービス、各種試薬・製造原料の販売をいたします。

ビジコムジャパンへのお問い合わせはこちら